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Differentiate Streptococcus pneumoniae from other streptococci using Oxoid™️ Optochin Disc. Optochin Discs are paper discs ready impregnated with Optochin (ethylhydrocuprein hydrochloride) which provide a convenient and reliable alternative to the bile-solubility test. Pneumococciare are sensitive to Optochin so that a culture shows a zone of inhibition around the impregnated disc, while streptococci either grow right up to the edge of the disc or, occasionally, show a very small zone of inhibition.