Meprobamate Cutoff Calibrator (100 ng/mL)

The LZI Carisoprodol Metabolite (Meprobamate) Enzyme Immunoassay is a homogeneous enzyme immunoassay ready-to-use liquid reagent. The assay is based on competition between meprobamate in the sample and the enzyme glucose-6-phosphate dehydrogenase (G6PDH) labeled with meprobamate for a fixed amount of antibody in the reagent (24). Enzyme activity decreases upon binding to the antibody, and the meprobamate concentration in the sample is measured in terms of enzyme activity. In the absence of meprobamate in the sample, meprobamate-labeled G6PDH conjugate is bound to antibody, and the enzyme activity is inhibited. On the other hand, when meprobamate is present in the sample, antibody would bind to free meprobamate; the unbound meprobamate-labeled G6PDH then exhibits its maximal enzyme activity. Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that can be measured spectrophotometrically at 340 nm.