A stable-labeled internal standard for tramadol testing or isotope dilution methods by GC/MS or LC/MS for applications in clinical toxicology, urine drug testing, pain prescription monitoring, or forensic analysis. Tramadol is a synthetic analgesic used to treat moderate to moderately severe pain. The drug, sold under trade names Ultram, Ultracet, Ralivia, and Tramal, has a wide range of applications from the treatment of rheumatoid arthritis to fibromyalgia.